Cytology

Cytology

  1. Where can I find instructions on how to collect a Gyn/Pap specimen?
  2. Where can I find instructions on how to collect an NGC/body fluid specimen?
  3. Can I use saline/rubbing alcohol/formalin to preserve a body fluid specimen?
  4. How do I order an HPV test?
  5. How do I know whether to do a pap test and/or HPV test?
  6. When can I expect my HPV result?
  7. How do I order CT/NG testing?
  8. Why am I getting "unsatisfactory results" on my patient's Pap test?
  9. How do I place an order in EPIC for cytology specimens?
  10. How can I order cytology and histology supplies?

  1. Where can I find instructions on how to collect a Gyn/Pap specimen?

    Click on the above link for written instructions and illustrations on how to collect a GYN/Pap specimen.

  2.  Where can I find instructions on how to collect an NGC/body Fluid specimen?

    Click on the above link for written instructions on how to collect different types of body fluid specimens.

  3.  Can I use saline/rubbing alcohol/formalin to preserve a body fluid specimen?

    The best procedure for preserving body fluid specimens is to use an equal volume of 50% ethyl alcohol added to the specimen.  If alcohol is not available, refrigeration of a fresh specimen is the next best method.

    Saline, rubbing alcohol, formalin or not using any preservation method can compromise the quality of the cells and may limit the processing options or make a diagnosis impossible.

  4.  How do I order an HPV Test?

    An HPV test can be ordered when originally submitting the Pap specimen, as a stand-alone test, or after the diagnosis of a Pap specimen has been received.

    HPV with Pap Specimen (Cotesting/Regardless of Diagnosis):  Collect a Pap Test specimen.  To request HPV testing at the same time as the Pap specimen, select "Regardless of diagnosis/co-testing" on the Cytology Request Form.

    HPV with a specific diagnosis:  Collect a Pap Test specimen.  On the Cytology Request Form, select the "Reflex HPV testing on" and the diagnosis (negative, ASC-US, ASC-H, AGC, LSIL, and/or HSIL) for which HPV will be run.  Multiple diagnoses may be selected.

    HPV only (no Pap specimen): Collect a Pap Test specimen.  To request HPV only with no Pap test, select "HPV Only (no Pap Test)" on the Cytology Request Form.

    HPV after the diagnosis of the Pap specimen has been received:  ThinPrep vials are kept for one week after a final diagnosis is rendered.  If an HPV test is required, submit a written request that provides, at a minimum, the patient name, date of birth, date of service and the test requested (i.e. "Please run HPV on this patient").  Please courier or fax the request to the lab at 715-847-0065.  If it is close to a week since the final report was issued, call the lab so that the vial can be pulled, but a written request is still needed, per CAP guidelines.

  5.  How do I know whether to do a PAP test and/or HPV Test?

    For current guidelines, refer to the Cervical Cancer Screening Guidelines for Average-Risk Women.  However, the final determination for requesting Pap and HPV tests should be based on the patient's history and the clinician's knowledge.

  6. When can I expect my HPV test result?

    HPV tests are processed in batched runs.  Depending on when the ThinPrep vial and/or the HPV test order is received in the lab, HPV test results should be available within seven business days.

  7.  How do I order CT/NG testing?

    To order these tests, select the appropriate option on the Cytology Order Requisition Form.  "Gonorrhea/Chlamydia" selections can be found on the Form directly below the HPV testing options.  It is not necessary to place an order in EPIC.                                                                        

  8.  Why am I getting "Unsatisfactory Results" on my patient's Pap test? 

 Unsatisfactory results are caused by:

  • Scant squamous epithelial component (well-preserved and well visualized cells covering less that 40% of the area on the slide)
  • Excessive blood, inflammation, mucus, lubricant or other material that replaces or obscures approximately 75% or more of the epithelial cells

To minimize unsatisfactory Pap test results:

  • Educate your patient on how they should prepare for a Pap test
  • Do not use lubricant during the collection procedure
  • Use proper technique when collecting a specimen
  • Reschedule a patient's Pap test for when they do not have their menstrual period.

   

     9.  How Do I place an order in EPIC for cytology Specimens?

        Please contact your EPIC EMR representative for specific instructions.

     10.  How can I order cytology and histology supplies? 

You can submit an order electronically by clicking here.  Complete the form and submit. 

You can call the Main Office number (715-847-0075) or the Cytology Laboratory directly (ext. 50075) to submit your supply order.

Your supply order should be filled within 24 hours and you should receive your supplies within 48 hours of ordering.